Major depressive disorder (MDD), commonly referred to as depression, is a result of faltering neurotransmitter function and chemical imbalances in the brain. Most antidepressants on the market target increasing serotonin levels because serotonin plays a key role in emotional processing, however they are not effective in treating every individual. Therefore, it is important to consider natural supplements, such as eicosapentaenoic acid (EPA) and L-tryptophan, that remedy neuropathic pain. In this study, the supplements EPA and L-tryptophan were tested on male and female Drosophila melanogaster to observe their behaviors signifying depression. D. melanogaster were fed the acids after depression was induced through sleep deprivation. Climbing assays were performed throughout the supplemental treatment to assess the efficacy of the acids. Negative geotaxis behavior was used as an indicator for the flies’ motivational state and depression-like symptoms during the climbing assays. While the data from the climbing assays is still being analyzed, successful treatment of MDD will be indicated through an increase of negative geotaxis behavior. This study is important because finding individualized and less invasive solutions for MDD is vital to combat the rising diagnoses of the disorder.
